Quantitation of Hepatitis B Virus Genomic DNA by Real-Time Detection PCR
نویسندگان
چکیده
منابع مشابه
Quantitation of hepatitis B virus genomic DNA by real-time detection PCR.
Quantitation of hepatitis B virus (HBV) DNA in serum is a useful method for the monitoring of HBV replication. We attempted to develop a quantitative assay system for HBV DNA that is more sensitive, accurate, and reproducible than existing systems. We detected HBV DNA by real-time detection PCR (RTD-PCR) based on Taq Man chemistry. The efficacy of this assay was evaluated by quantitatively meas...
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Background Both real-time-polymerase chain reaction (PCR) and hybrid capture 2 (HC2) assay can detect and quantify hepatitis B virus (HBV) DNA. However, real-time-PCR can detect a wide range of HBV DNA, while HC2 assay could not detect lower levels of viremia. The present study was designed to detect and quantify HBV DNA by real-time-PCR and HC2 assay and compare the quantitative data of these ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1999
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.37.9.2899-2903.1999